Purity：SDS-PAGE

Anti-GPC3 / Glypican-3 Reference Antibody (codrituzumab) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.

Puity：SEC-HPLC

The purity of Anti-GPC3 / Glypican-3 Reference Antibody (codrituzumab) is more than 95%, determined by SEC-HPLC.

Bioactivity：FACS

Human GPC3 CHOS cells were stained with Anti-GPC3 / Glypican-3 Reference Antibody (codrituzumab) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC50=2.142 nM.

Bioactivity：FACS

HEP3B cells were stained with anti-GPC3 / Glypican-3 Reference Antibody (codrituzumab) and negative control protein respectively, washed and then followed by PE and analyzed with FACS.

Bioactivity：FACS

HEPG2 cells were stained with anti-GPC3 / Glypican-3 Reference Antibody (codrituzumab) and negative control protein respectively, washed and then followed by PE and analyzed with FACS.

Bioactivity：ELISA

Immobilized human GPC3, His Tag at 2 ug/mL can bind Anti-GPC3 / Glypican-3 Reference Antibody (codrituzumab), EC50=0.008741 μg/mL.

Function：ADCC

Anti-GPC3 / Glypican-3 Reference Antibody (Codrituzumab) induced ADCC activity was evaluated using human GPC3 CHOS Cell. The max Lysis rate was approximately 30%.

Function：Internalization

The endocytosis ratio codrituzumab by HuH-7 increased with the increase of antibody concentration, and the Internalization Rate (%) reached 40%at antibody concentration of 1 nM.

Bioactivity：FACS

Huh-7 cells were stained with anti-GPC3 / Glypican-3 Reference Antibody (codrituzumab) and negative control protein respectively, washed and then followed by PE and analyzed with FACS.

-25~-15℃保存，有效期1年。