The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants.

Western

Western blot analysis of Glutamine Synthetase in Jurkat (1), and mouse liver (2) cell lysate using Glutamine Synthetase antibody.

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded human cerebellar tissues using Glutamine Synthetase antibody.

Immunocytochemistry

Immunocytochemistry analysis of Glutamine Synthetase in hela cells using Glutamine Synthetase antibody (green),and DAPI(blue). Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Flow

Flow Cytometry analysis of Hela cells using Glutamine Synthetase antibody (green) and negative control (red).