Mutations in Doublecortin cause Lissencephaly (smooth brain), a neuronal migration disorder characterized by epilepsy and mental retardation. Doublecortin is a microtubule associated protein that stabilizes and bundles microtubules. A conserved doublecortin domain mediates the interaction with microtubules, and interestingly most missense mutations cluster in this domain.

WB: 1/500-1/1000  IHC: 1/100-1/200  IF: 1/50-1/200  FC: 1/50-1/100

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded brain tissue tissues using Doublecortin antibody with DAB staining.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunohistochemical

Immunohistochemical analysis of paraffin-embedded Human tonsils using Doublecortin antibody, with DAB staining.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunofluorescence

Immunofluorescence analysis of Doublecortin in HepG2 cells using Doublecortin antibody (green). Blue: DRAQ5 fluorescent DNA dye.

Flow

Flow cytometry analysis of SK-N-SH stained with DCX antibody (green) and negative control (red).

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