E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity.

推荐稀释比 WB:1/500-1/1000;IP:1/20

Western blot analysis of NEDD4 in K562, HCT116, 3T3, C6 lysates using NEDD4 antibody.

Western blot analysis of NEDD4 in Hela, A549, HL-60, U2OS, U87-MG lysates using NEDD4 antibody.

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.