Numb acts as a negative regulator of Notch signaling by promoting ubiquitination and degradation of Notch. The protein is asymmetrically segregated into one daughter cell during cell division, producing two daughter cells with different responses to Notch signaling and different cell fates. The localization of Numb can also be regulated by G-protein coupled receptor (GPCR) and PKC signaling.

推荐稀释比 WB:1/500-1/1000;IHC:1/50-1/100;IP:1/20

Western blot analysis of NUMB in 3T3, C6, rat Brain, MCF-7 lysates using NUMB antibody.

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using NUMB antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Store at 4°C short term. Aliquot and store at -50°C long term. Avoid freeze/thaw cycles.