细胞核蛋白、细胞浆蛋白在细胞研究和蛋白质组学中具有重要意义，因此在体外研究中需要将其从细胞或组织中分离。本试剂盒提供了一种较为简单方便，高效的蛋白抽提方法，其原理是：通过细胞浆蛋白抽提试剂A和B，在低渗透压条件下，使细胞充分膨胀，然后破坏细胞膜，释放出细胞浆蛋白，然后通过离心得到细胞核沉淀。最后通过高盐的细胞核蛋白抽提试剂C抽提得到细胞核蛋白。
本试剂盒1次可对2×106个细胞和30-50 mg组织样品进行抽提，按照该使用量，20126ES50可抽提50个样品，20126ES60可抽提100个样品。
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产品组分信息
| 组分编号 | 组分名称 | 20126ES50(50T) | 20126ES60(100T) |
|---|---|---|---|
| 20126-A | Cytoplasmic Extraction Reagent A 胞浆蛋白抽提试剂A | 15 mL | 30 mL |
| 20126-B | Cytoplasmic Extraction Reagent B胞浆蛋白抽提试剂B | 0.5 mL | 1 mL |
| 20126-C | Nuclear Extraction Reagent 胞核蛋白抽提试剂 | 2.5 mL | 5 mL |
1.即用型试剂，操作简单快捷。
2.能够分离不同细胞定位的蛋白。
冰袋运输。-15~-25℃保存，1年有效。
Q：组织提取浆蛋白，组织量与提取试剂的比例如何？
A：将组织切成非常细小的碎片。根据后续使用量按照20:1的比例混合试剂A和B，并加入PMSF至最终浓度为1 mM，混匀即可配制成组织匀浆液。按照每60 mg组织加入200 µL组织匀浆液的比例混合组织和组织匀浆液（对于不超过60mg的组织样品，仅需加入100微升组织匀浆液），并在玻璃匀浆器内充分匀浆。接下来请参照【细胞样品蛋白抽提】的步骤2）开始操作，即把沉淀当做已经离心收集好的细胞沉淀操作。（特别说明：对于起始量为60mg的组织样品，后续直接按照步骤2-7操作即可；对于起始量不超过60mg的组织样品，后续步骤中的溶液的用量须减半使用)。对于组织样品，如果每个样品的重量不超过60mg，本试剂盒可以抽提50个样品，如果每个样品为60mg，本试剂盒可以抽提25个样品。
Q：该产品是否可以用于冻存组织？
A：对于组织样品，仅适合于新鲜组织，对冻存过的组织抽提效果很差。
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