MitoTracker® Green FM是一种细胞渗透型的carbocyanine结构衍生物，包含标记线粒体的弱巯基反应性的氯甲基官能团。本品是一种亮绿色荧光探针（（Ex=490 nm，Em=523 nm）），其在水溶液中几乎不发荧光，只有当聚集在线粒体的脂质环境才能发荧光，背景荧光几乎可忽略。使得操作步骤极其简单，只需用探针孵育细胞，即可被动运输穿过细胞膜并直接聚集在活性线粒体上，可不经过清洗直接观察。
与MitoTracker® Red CMXRos不同的是本品定位于线粒体的能力不受线粒体膜电位影响，使其可能作为定量线粒体质量的一种工具。本品经乙醛固定后染料不稳定，因此更适合活细胞染色。
虽然传统的线粒体荧光探针如TMR和罗丹明123，也能很容易的聚集在功能线粒体上，但是一旦线粒体膜电位丧失即会被洗掉，从而在一些需要细胞进行醛类固定或者包含线粒体能量状态影响因子的实验中，使其应用大受限制。
| CAS号（CAS NO.） | 201860-17-5 |
| 分子式（Formula） | C34H28Cl5N3O |
| 分子量（Molecular weight） | 671.88 |
| 外观（Appearance） | 红色固体 |
| 激发波长（Ex） | 490 nm |
| 发射波长（Em） | 523 nm |
| 结构式（Structure） |  |
室温运输； -20℃避光干燥保存，有效期两年。
Q：没有 HBSS 用什么缓冲液，加多少体积上流式？
A：可以使用 PBS 或是无血清培养基替代，添加体积足够做流式即可 100-200ul，
Q：染色结果可以使用多功能酶标仪观察吗？
A：可以，建议如果条件允许最好是使用荧光显微镜或是共聚焦显微镜 流式仪器。
Q：我可以在酶标仪上使用 MitoTracker Red CMXRos 染料来研究线粒体膜电位变化吗？
A：是的，已经有很多文献介绍。 但是，请注意酶标仪通常不如荧光显微镜或流式细胞仪那么敏感，
这意味着荧光变化的程度可能不像酶标仪那样敏感或容易检测到。确保仔细优化标签时间和浓度.
Q：40740ES 和 40741ES有什么区别？
A：40740ES50 是 40741ES50 的非荧光还原态，在氧化时才会发出荧光， 该染料也可以对活细胞线
粒体进行染色并且其积累取决于膜电位。乙醛固定后，该染料可稳定保存。
Q：我的 Mitotracker 染色结果看上去不仅仅是染色线粒体。 为什么？
A：这通常是使用浓度过高的 Mitotracker 染料的结果，大多数有机染料应在低浓度范围内使用。
MitoTracker 染料的使用浓度非常低，约为 50-200n M，较高的浓度会导致背景荧光和非线粒体染色结果的出现。
Q：线粒体探针的保质期是多久？
A：按规定标准储存，保质期一年
Q：是否受膜电位的影响，如果不受膜电位影响能否固定后染色？或者染色后固定？
A：这个染料不受膜电位的影响，只能染活细胞，不兼容固定后染色和染色后固定。若活细胞染色后固定会导致荧光信号丢失。
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