Fluoromount-G™是一种不含有荧光探针的水溶性封片剂,用来对最后一步染色是在水溶液体系中完成的玻片样本进行封片,可以显著减缓各种常见荧光染料的荧光淬灭现象。本封片剂具有半永久性封固性,可用作长期保存玻片样本的制作。适用于细胞制备物和组织切片的封片,兼容于免疫荧光,免疫细胞化学,免疫组化等实验。
本品是一种无色透明溶液,室温保存稳定性高。封片剂干燥后形成一层牢固的涂层,光学透明,便于后续荧光观察和拍照。
室温运输和保存即可,一年有效。
Q:该产品需要低温储存吗?
A:不需要,室温运输和保存即可。
Q:制备好玻片能够长久保存吗?
A:若玻片样本需长久保存,可使用指甲油或者中性树胶对盖玻片四周进行密封。
Q:使用该产品后静置多久?
A:室温下避光静置 5 min,使得封片剂晾干后,再进行普通荧光显微镜或者共聚焦显微检测。
Q:该产品可用于免疫荧光,免疫细胞化学,免疫组化实验吗?
A:可以。
[1] Lu Y, Li Y, Liu Q, et al. MondoA-Thioredoxin-Interacting Protein Axis Maintains Regulatory T-Cell Identity and Function in Colorectal Cancer Microenvironment. Gastroenterology. 2021;161(2):575-591.e16. doi:10.1053/j.gastro.2021.04.041(IF:22.682)
[2] Zhao D, Yang F, Wang Y, et al. ALK1 signaling is required for the homeostasis of Kupffer cells and prevention of bacterial infection. J Clin Invest. 2022;132(3):e150489. doi:10.1172/JCI150489(IF:14.808)
[3] Nie M, Yang L, Bi X, et al. Neutrophil Extracellular Traps Induced by IL8 Promote Diffuse Large B-cell Lymphoma Progression via the TLR9 Signaling. Clin Cancer Res. 2019;25(6):1867-1879. doi:10.1158/1078-0432.CCR-18-1226(IF:8.911)
[4] Zhang M, Bai Y, Xu C, et al. Blockage of Extracellular Signal-Regulated Kinase Exerts an Antitumor Effect via Regulating Energy Metabolism and Enhances the Efficacy of Autophagy Inhibitors by Regulating Transcription Factor EB Nuclear Translocation in Osteosarcoma. Front Cell Dev Biol. 2021;9:650846. Published 2021 Aug 3. doi:10.3389/fcell.2021.650846(IF:6.684)
[5] Zhang M, Lin J, Jin J, Yu W, Qi Y, Tao H. Delivery of siRNA Using Functionalized Gold Nanorods Enhances Anti-Osteosarcoma Efficacy. Front Pharmacol. 2021;12:799588. Published 2021 Dec 20. doi:10.3389/fphar.2021.799588(IF:5.811)
[6] Liu S, Li YM, Li JZ, et al. CD4+ T Cells Promote IgG Production in MHC-Independent and ICAM-1-Dependent Manners in Pristane-Induced Lupus Mice. Mediators Inflamm. 2022;2022:9968847. Published 2022 Jan 21. doi:10.1155/2022/9968847(IF:4.711)
[7] Li T, Mei Y, He B, Sun X, Li J. Reducing Quorum Sensing-Mediated Virulence Factor Expression and Biofilm Formation in Hafnia alvei by Using the Potential Quorum Sensing Inhibitor L-Carvone. Front Microbiol. 2019;9:3324. Published 2019 Jan 9. doi:10.3389/fmicb.2018.03324(IF:4.259)
[8] Liu C, Wang JO, Zhou WY, et al. Long non-coding RNA LINC01207 silencing suppresses AGR2 expression to facilitate autophagy and apoptosis of pancreatic cancer cells by sponging miR-143-5p. Mol Cell Endocrinol. 2019;493:110424. doi:10.1016/j.mce.2019.04.004(IF:4.102)
[9] Zhao SQ, Shen ZC, Gao BF, Han P. microRNA-206 overexpression inhibits epithelial-mesenchymal transition and glomerulosclerosis in rats with chronic kidney disease by inhibiting JAK/STAT signaling pathway. J Cell Biochem. 2019;120(9):14604-14617. doi:10.1002/jcb.28722(IF:3.448)
[10] Zhang H, Lv H, Weng M, et al. Preoperative leukocytosis is associated with increased tumor-infiltrating neutrophil extracellular traps and worse outcomes in esophageal cancer. Ann Transl Med. 2020;8(7):441. doi:10.21037/atm.2020.03.190(IF:3.297)
[11] Zhang M, Xia L, Yang Y, et al. PD-1 blockade augments humoral immunity through ICOS-mediated CD4+ T cell instruction. Int Immunopharmacol. 2019;66:127-138. doi:10.1016/j.intimp.2018.10.045(IF:3.118)
[12] Yu W, Shi X, Cui X, et al. Jian-Pi-Zhi-Dong-Decoction regulates the expression of glutamate transporters to attenuate glutamate excitotoxicity and exerts anti-tics effects in Tourette syndrome model rats. Neuropsychiatr Dis Treat. 2018;14:3381-3392. Published 2018 Dec 17. doi:10.2147/NDT.S185169(IF:2.195)