DiI, DiO, DiD, DiR是一系列亲脂性的荧光染料，可以用来染细胞膜和其它脂溶性生物结构。这些环境敏感性荧光染料在进入细胞膜之前荧光非常弱，当与细胞膜结合后其荧光强度大大增强。且具有消光系数高、极性依赖性、激发态寿命短等特点。一旦进入细胞膜后，可在整个细胞膜上扩散，最佳浓度时可以使整个细胞膜染色。不同的荧光染色（DiI:橙色荧光; DiO:绿色荧光; DiD:红色荧光;DiR:深红色荧光）为活细胞多标、流式分析提供了便利：1）DiO和DiI可分别用标准的FITC滤光片和TRITC滤光片检测。2）DiD是DiI的衍生物，具有明显往红光迁移的激发和发射光谱，这一特性使其特别适合标记具显著自荧光效应的细胞或组织。3）DiR具强效的近红外光穿透细胞或组织，并在近红外区具较低的自荧光水平，这些特性使其特适用于体内成像或示踪实验。

冰袋（wet ice）运输。产品-20 ºC干燥避光保存，有效期一年。

Q：这些探针染细胞，有没有细胞特异性？会不会 293 细胞不好染， MSC 好染？

A：由于这些染料是亲脂性染料，是通过插入到脂质膜中进而被激光激发观察，对于动植物细胞没有

特异性。

Q：这些探针荧光探针装载完多久后观察？

A：为减少各种可能的误差，尽量缩短探针装载后到仪器测定所用的时间(刺激时间除外)。

Q：这些探针染色细胞后，对细胞有毒性吗？

A：荧光探针类产品对活细胞都会有一定的毒性，因此建议在染色处理完成后应尽快完成检测和观察。

Q：为什么染色后发现荧光颜色较浅，应怎么操作进行改进？

A：可以尝试增加染色的时间和浓度。

Q：要看几个小时过程中活细胞的形态变化，应该怎么选？

A：通常使用亲脂性花青染料，如 DiI，DiO，DiD 或 DiR。

Q：DiRIodide(DiIC18(7))该探针在进行HPLC检测时该如何设置？

A：请使用正向色谱柱； 氯仿/甲醇～10:1。

（部分高分文献节选）

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