ER-Tracker探针是一系列具有细胞膜透过性,对活细胞内质网具有高度选择性的探针,不像传统的DiOC6(3),该系列探针几乎不对线粒体着色,且在较低浓度即可实现对内质网的染色,且对细胞几乎无毒性。使用以下提供的优化步骤对活细胞染色后用醛固定,可以部分保留活细胞的染色特征。
本品为ER-Tracker Blue-White DPX,Ex= 374 nm,Em= 430-640 nm,蓝色荧光,属于DapoxylTM(DPX)家族成员之一。DPX系列染料均具有长发射波长、高吸光系数、高量子产量、较大斯托克司频移等特点。但这些染料具有环境敏感性,会随之出现极性增强、最大荧光波长偏移到更高波长处、量子产量下降等现象。
本品为溶于DMSO的1 mM ER-Tracker Blue-White DPX储存液,推荐工作浓度为100 nM-1 μM。
分子式(Formula) | C26H21F5N4O4S |
分子量(Molecular Weight) | 580.53 |
外观(Appearance) | Yellow Solution |
Ex/Em | 374/430-640 nm |
滤片(Filter) | DAPI or UV longpass |
结构式(Structure) | ![]() |
冰袋运输。≤-20℃避干燥保存,一年效期。避免反复冻融。
[1] Zhou C, Li J, Du J, et al. HMGCS1 drives drug-resistance in acute myeloid leukemia through endoplasmic reticulum-UPR-mitochondria axis. Biomed Pharmacother. 2021;137:111378. doi:10.1016/j.biopha.2021.111378(IF:6.530)
[2] Guo X, Lu D, Zhang D, et al. Curved corannulene dually targets mitochondria and endoplasmic reticulum, and initiates apoptosis via localized ROS induction upon light triggering. Mater Sci Eng C Mater Biol Appl. 2020;106:110227. doi:10.1016/j.msec.2019.110227(IF:4.959)
[3] Zhao M , Gao Y , Ye S , et al. A light-up near-infrared probe with aggregation-induced emission characteristics for highly sensitive detection of alkaline phosphatase. Analyst. 2019;144(21):6262-6269. doi:10.1039/c9an01505a(IF:4.019)
[4] Wang XH, Yin S, Ou XH, Luo SM. Increase of mitochondria surrounding spindle causes mouse oocytes arrested at metaphase I stage. Biochem Biophys Res Commun. 2020;527(4):1043-1049. doi:10.1016/j.bbrc.2020.05.037(IF:2.985)