Polybrene（聚凝胺，hexadimethrine bromide）是一种多聚阳离子聚合物，常用于哺乳动物细胞的DNA转染实验以增强脂质体的转染效率。Polybrene目前广泛用于逆转录病毒介导的基因转染，慢病毒介导的基因转染，作用机理可能是通过中和细胞表面唾液酸与病毒颗粒之间的静电排斥从而促进吸附作用。Polybrene也是一种有名的抗肝素剂（肝素拮抗剂），常用来生产非特异性凝集的红细胞。另外Polybrene也多用于蛋白测序，因为小剂量的Polybrene在自动测序分析可明显改善多肽的降解现象。PVDF膜加入polybrene还能提高膜的亲和性。
本产品以溶液形式提供，粉末用0.9% NaCl配制成10 mg/mL的溶液，并用0.22 μM滤膜过滤除菌。使用时一般按1:1000-1:2000稀释，依细胞种类不同稀释比例不同，具体查阅相关文献。
提高转染效率：Polybrene（聚凝胺）是一种阳离子聚合物，能够与病毒载体（如慢病毒）表面的负电荷相互作用，增加病毒颗粒对细胞的黏附，从而显著提高病毒对细胞的感染效率。
稳定性好：该产品在2-8℃的条件下保存，性能稳定，有效期较长，能够保证在实验过程中提供可靠的效果。
操作简便：使用时无需复杂的预处理，可以直接按照一定的比例加入到含有病毒载体的培养基中，与其他转染试剂或方法相比，操作步骤相对简单。
冰袋（wet ice）运输。-20 ºC可保存2年，建议分装保存，避免反复冻融。
Q: 聚凝胺浓度是10mg/ml，用什么稀释？
A: 聚凝胺稀释不是指用其他溶液稀释，而是指在具体实验中加入的比例，比如实验体系有10mL,按照这个比例（1：1000为例），就直接取10uL的产品（10mg/mL溶液）加入实验体系里面即可。
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