细胞在发生凋亡时，会激活一些DNA内切酶，这些内切酶会切断核小体间的基因组DNA。细胞凋亡时抽提DNA进行电泳检测，可以发现180-200 bp的DNA ladder。
TUNEL (TdT mediated dUTP Nick End Labeling) 细胞凋亡检测试剂盒（YSFluorTM 640）可以用来检测组织细胞在凋亡晚期过程中细胞核DNA的断裂情况。其原理是在末端脱氧核糖核苷酸转移酶（Terminal Deoxynucleotidyl Transferase, TdT）的作用下，在基因组DNA断裂时暴露出的3´-羟基（3´-OH）末端掺入YSFluorTM 640-12-dUTP，从而可以用荧光显微镜或流式细胞仪检测。YSFluorTM 640为红色荧光染料，具有很高的稳定性和很强的亮度。
应用范围广，可以用于检测冷冻或石蜡切片中的细胞凋亡情况，也可以检测培养的贴壁细胞或悬浮细胞的凋亡情况。
冰袋（wet ice）运输。
本试剂盒储存在-20℃，YSFluorTM 640-12-dUTP Labeling Mix避光储存于-20℃，保质期为1年。
Q：血管内皮细胞，想要免疫荧光和 TUNEL 同时进行，不知道该先做哪个? TUNEL 要求立即检测？
A：先做 TUNEL，再进行免疫荧光，这个主要是怕免疫荧光对 TdT 酶产生影响。只要避光保存就可以不立即进行检测。
Q：感觉 DNA 染的有点弥散状，核定位的效果也不是很好，是染色时间太久？感觉细胞质中也像是染到了？
A：有非特异性染色，可能是因为时间太久或者是试剂浓度太高导致的。建议降低染色液浓度或减少染色时间。
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