Hifair® Ⅲ One Step RT-qPCR SYBR Green Kit是以RNA为模板进行定量PCR反应的试剂盒。在实验的过程中，逆转录和定量PCR在同一反应管中进行，简化了实验操作，降低了污染的风险。本试剂盒以RNA为模板，使用基因特异性引物，利用耐热Hifair® Ⅲ Reverse Transcriptase高效合成第一链cDNA，并以此为模板在UNICON® HotStart Taq DNA Polymerase作用下，配合优化的缓冲体系能够特异灵敏的对RNA模板进行精确定量。同时Buffer中额外添加了有效抑制非特异性PCR扩增的组分和提高扩增能力的促进因子，使定量PCR结果可以在宽广范围内更加真实有效。
本试剂盒以Kit形式提供，2×Hifair® Ⅲ SG buffer包含优化的缓冲体系和dNTPs等，优化了有效抑制非特异性PCR扩增的因子和提升PCR反应扩增效率的因子，适用于SYBR Green标记的高特异性检测系统。此外，Hifair® UH Ⅲ Enzymes包含比例优化的Hifair® Ⅲ Reverse Transcriptase、RNase inhibitor及UNICON® HotStart Taq DNA Polymerase等。
产品组分
| 组分编号 | 组分名称 | 11143ES50 | 11143ES70 | 11143ES80 |
|---|---|---|---|---|
| 11143-A | 2×Hifair® Ⅲ SG buffer | 500 μL | 4×500 μL | 5 mL |
| 11143-B | Hifair® UH Ⅲ Enzymes | 50 μL | 200 μL | 500 μL |
| 11143-C | Hieff® 50×High Rox | 50 μL | 200 μL | 500 μL |
| 11143-D | Hieff® 50×Low Rox | 50 μL | 200 μL | 500 μL |
| 11143-E | RNase-free ddH2O | 500 μL | 4×500 μL | 5 mL |
- 检出率：大于90%
- 扩增效率：大于90%
- 特异性强：无非特异性扩增
- 检测灵敏度高：>10pg total RNA
- 保存稳定性：反复冻融>50次buffer与酶分开，buffer冻融稳定
- 灵敏度高：GAPDH能够检测到低至2pg的投入量
- 其它来源模板测试，CT值小于竞品，且扩增产物单一
-25~-15℃避光储存，有效期1年。本品避免反复冻融。建议分装保存。
Q：产品的应用领域和特点是什么？
A：应用于病毒检测，特点是高检出率
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