Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small HSPs, HSP27 is regulated at both the transcriptional and posttranslational levels. In response to stress, the HSP27 expression increases several-fold to confer cellular resistance to the adverse environmental change. HSP27 is phosphorylated at Ser15, Ser78, and Ser82 by MAPKAPK-2 as a result of the activation of the p38 MAP kinase pathway.

Western

Western blot analysis of Phospho-Hsp27 (Ser82) in HL-60 lysates untreated or treated with UV using HSP27 (Phospho-Ser82) antibody.

Western

Western blot analysis of Phospho-Hsp27 (Ser82) in Hela cells, untreated or treated with UV, using HSP27 (Phospho-Ser82) antibody (upper) or Hsp27 antibody(lower).

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma tissue using HSP27 (Phospho-Ser82) antibody (left) or the same antibody preincubated with blocking peptide (right).High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.